Hydrophilic interaction liquid chromatography (HILIC) in proteomics
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چکیده
منابع مشابه
Hydrophilic interaction liquid chromatography (HILIC) in proteomics
In proteomics, nanoflow multidimensional chromatography is now the gold standard for the separation of complex mixtures of peptides as generated by in-solution digestion of whole-cell lysates. Ideally, the different stationary phases used in multidimensional chromatography should provide orthogonal separation characteristics. For this reason, the combination of strong cation exchange chromatogr...
متن کاملHydrophilic interaction liquid chromatography (HILIC)—a powerful separation technique
Hydrophilic interaction liquid chromatography (HILIC) provides an alternative approach to effectively separate small polar compounds on polar stationary phases. The purpose of this work was to review the options for the characterization of HILIC stationary phases and their applications for separations of polar compounds in complex matrices. The characteristics of the hydrophilic stationary phas...
متن کاملChromatographic analysis of olopatadine in hydrophilic interaction liquid chromatography.
In this paper, chromatographic analysis of active substance olopatadine hydrochloride, which is used in eye drops as antihistaminic agent, and its impurity E isomer by hydrophilic interaction liquid chromatography (HILIC) and application of design of experiments (DoE) methodology are presented. In addition, benzalkonium chloride is very often used as a preservative in eye drops. Therefore, the ...
متن کاملHydrophilic Interaction Chromatography (HILIC) Separation of Basic Drugs using MS/MS Detection Application
The basic drugs, ranitidine and paroxetine were successfully separated using hydrophilic interaction liquid chromatography (HILIC). The elution order was reversed compared to reversed-phase liquid chromatography (RPLC). Good linearity was shown over the 0.5–100 ppb levels. A spiked serum was cleaned up by protein precipitation and an aliquot was directly injected into the HILIC/ESI-MS system. R...
متن کاملHydrophilic interaction chromatography (HILIC) for LC-MS/MS analysis of monoamine neurotransmitters.
BACKGROUND Hydrophilic interaction chromatography (HILIC) is becoming an increasingly popular alternative to traditional reversed-phase chromatography for the analysis of polar compounds. The ability to retain the most polar compounds in HILIC makes it attractive for the analysis of certain large groups of compounds, such as monoamines, which are inherently very polar. RESULTS This paper deta...
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ژورنال
عنوان ژورنال: Analytical and Bioanalytical Chemistry
سال: 2008
ISSN: 1618-2642,1618-2650
DOI: 10.1007/s00216-008-1865-7